early passage normal human female colon fibroblast ccd 18co cell line  (ATCC)

Journal: Foods

Article Title: Valorization and Functional Enhancement of Mature Assam Tea Leaves Through Indigenous Filamentous Fungi-Based Fermentation for Functional Drink Development

doi: 10.3390/foods15091562

Figure Lengend Snippet: The cytotoxicity of Caco-2 cells ( A ) and HT-29 cells ( B ) after treatment with co-fermented mature tea leaves samples for 72 h. Different lowercase letters indicate significant differences among fermentation times within the same treatment ( p < 0.05).

Article Snippet: Human colon carcinoma cell line (Caco-2) and Human colon adenocarcinoma cell line (HT-29) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) were used to carry out the cell cytotoxicity test.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: The cytotoxic effect of Myr-B peptide against human colon cancer Caco-2 cells. Cell viability was assessed using the MTT assay for the Caco-2 cell line after 3 h and 24 h of incubation with N-myristoylated Myr-B peptide ( a ) and corresponding non-myristoylated Pep-B peptide ( b ). Data are expressed as a percentage of viable cells in the presence of different peptide concentrations (2.5–50 µM) compared to untreated Caco-2 cells (negative control). Positive control represents cells treated with 2% NaN 3 . Untreated cells serve as negative control. All experiments were performed using two independent replicates, each with at least three repeats. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s post hoc test (GraphPad Prism 11.0). Difference from the negative control was considered statistically significant as follows: ** adjusted p -value < 0.01; **** p -value < 0.0001.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: MTT Assay, Incubation, Negative Control, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: Evaluation of Myr-B peptide-induced death of human cervical cancer HeLa ( a ) and colon cancer Caco-2 ( b ) cells. Cell membrane integrity was assessed through lactate dehydrogenase (LDH) release from untreated HeLa or Caco-2 cells (negative control) after 3 h exposure of HeLa and Caco-2 cells to their respective IC 50 doses of Myr-B peptide (38 μM and 50 μM, respectively) and from HeLa or Caco-2 cells treated with a lysis solution to release all LDH (positive control). The exposure to Pep-B was carried out under the same conditions as the corresponding treatment with the Myr-B peptide for each cancer cell line. Statistical significance was assessed using one-way ANOVA followed by Dunnett’s post hoc test (GraphPad Prism 11.0). Difference from the negative control was considered statistically significant as follows: * adjusted p -value < 0.05; *** p -value < 0.001; **** p -value < 0.0001.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: Membrane, Negative Control, Lysis, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Anticancer Activity of the Antimicrobial Myristoylated Peptide Myr-B in HeLa Cells: Cytotoxic, Membrane-Disruptive and Proteomic Insights

doi: 10.3390/ijms27093918

Figure Lengend Snippet: SEM analysis of Myr-B peptide-induced effects on human colon cancer Caco-2 cells. SEM micrographs (acquired and processed using JEOL inTouchScope Interface software) of untreated Caco-2 cells (negative control) ( a , b ), Caco-2 cells after 3 h exposure to 50 μM Myr-B ( c , d ) and 50 μM Pep-B ( e , f ) and Caco-2 cells treated with 2% NaN 3 (positive control) ( g , h ). Magnification and scale bars = ( a – d ) 1500× and 10 μm, respectively; ( e – h ) 10,000× and 1 μm, respectively.

Article Snippet: The human colon cancer Caco-2 cell line (ATCC HTB-37) was cultured in DMEM supplemented with 10% ( v / v ) FBS, 1% ( w / v ) glutamine, 1% ( w / v ) penicillin–streptomycin, 1% Non-Essential Amino Acids and 1% sodium pyruvate and maintained at 37 °C in a humidified incubator with 5% CO 2 [ ].

Techniques: Software, Negative Control, Positive Control